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Description

Ca 2+ promotes the translocation of ALS-related RNA-binding proteins from the nucleus to the cytoplasm in primary cortical neurons and MNs

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For TBC1D9B KD, reverse transfection was similarly performed, with a subsequent repeat after 24 h, utilizing 100 nM siRNA and jetPRIME, following the manufacturers instructions

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The supernatants were transferred to clean tubes, then held on ice until the end of the experiment, and stored at 4 C

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